The absorbance was read at 20 min intervals for 2 h at 470 nm. Phenolic metabolites seem to be contributing significantly towards antioxidant activity of the c. Mountain papayas carica pubescens spread limitedly and can only adapt in the. Papaya seeds are discarded after consuming the fruit, but they contain many bioactive compounds. Papaya seeds were extracted in methanol and hexane. According to the procedure, to 1 ml of ethanolic extract solution 25 gml, added 5 ml methanolic solution of 2,2. An overview vijay yogiraj, pradeep kumar goyal, chetan singh chauhan, anju goyal, bhupendra vyas abstract papaya carica papaya linn is commonly called as pawpaw and it belongs to the family caricaceae. Antibacterial activity of seed and leaf extract of carica papaya var. Antioxidant potential of carica papaya peel and seed. Ananas comosus, artocarpus heterophyllus, carica papaya, citrullus vulgaris, citrus sinensis, malus domestica, manilkara zapota, musa paradisiaca, phyllanthus emblica, psidium guajava and pyrus communis.
Thin layer chromatographic analysis and antioxidant. The 11 fruits studied had comparable antioxidant activity in both, abts and dpph assays. Sd on the phytochemicals composition and antioxidant activities of carica papaya seeds, a medicinal plant, was investigated. Papaya as an alternative antibiotic for the development of newer antibacterial agents. Antioxidant analysis of total phenol, total flavonoid, and ferric reducing antioxidant property frap on aqueous extracts of unripe papaya seed and peel. The efficacy of papaya leaves has been studied in reducing cardiovascular disease keywords active packaging active substance antimicrobial antioxidant papaya leaves article history received. The antioxidant properties were determined by 2,2diphenyl1picrylhydrazyl dpph, ferric reducing antioxidant. Antioxidant analysis of different parts of carica papaya. The antioxidant activities of different parts of papaya extract were assayed based on the. Fermented papaya preparation fpp is a product of yeast fermentation of carica papaya linn. Carica papaya is a well known medicinal plant used in the west and asian countries to cope several diseases. The cells were washed with 1 x pbs twice and incubated with 1. Antiinflammatory and antioxidant properties of unripe papaya.
Pdf the antioxidant activities of the ethanol, petroleum ether, ethyl acetate. The ethyl acetate fraction showed the strongest dpph and hydroxyl free radicalscavenging activities, and its activities were stronger than those of ascorbic acid and sodium benzoate, respectively. The antioxidant effect of fermented papaya preparation in the. Antioxidant activity of carica papaya leaf extract. It is an herbaceous plant that grows in tropical and sub tropical countries 20. Antioxidant effect of aqueous carica papaya seeds extract. Thus papaya seeds offered protection to cells from oxidative stress.
Ripe papaya is most commonly consumed as fresh fruit whereas green papaya as vegetable usually. Papaya peels pp are discarded after consuming the fruit. Evaluation of antioxidant properties in fresh and pickled papaya. The antioxidant activity of extracts was based upon. Introduction cancer is a leading cause of death worldwide, it is a dreadful disease, and combating this disease is of great importance to public health. Papaya fruits extracts enhance the antioxidant capacity and. The purpose of this paper is to investigate various extracts of annona squamosa l annonanceae and carica papaya l caricaceae seeds for their antioxidant activity, free radical. Influence of processing in two cultivar of carica papaya from. Effect of drying methods on the phytochemicals composition. Antioxidant activity by dpph method antioxidant activity was determined by measuring ic 50 of dpph scavenging activities in three different processing of two cultivar of papaya using dpph assays were shown in fig 1 and fig 2. Antioxidants are a special group of nutrients produced by the cell, which removes supplements that scavenge free radicals 9. Molecules free fulltext antioxidant activity of papaya. The objective of this study was to determine the invitro antioxidant activity of papaya seeds and their effect on endogenous antioxidant enzymes in hepg2 cells.
Papaya plants contain karpain, a substance that kills microorganisms that often interfere with the digestive function. Tpc was determined by folinciocalteu method, while antioxidant activities. The result further showed that total flavonoid, total phenolic content and antioxidant activity of the dried leaves of moringa oleifera, mangifera l and carica papaya lare significantly higher than those of the fresh leaves. Administration dependent antioxidant effect of carica papaya. Invitro antioxidant activities of ocimum gratissimum, vitex doniana, carica papaya and peristrophe bicalyculata using dpph free radical scavenging activity. Free radical scavenging activity was determined according to the elimination of dpph radicals and it was found that methanol extracts of c. Such effects are attributed to the phenolic compounds and alkaloids present in the pulp, seed and peel extracts of c.
The present study aimed to evaluate the antioxidative potential of selected carica papaya peel and seed by determining their total phenolic content tpc and antioxidant activities. Topical agents which have antioxidant properties improve wound healing significantly and protect tissues from oxidative damage roy et al. Effects of drying on total polyphenols content and. Antiinflammatory and immunomodulatory properties of carica papaya saurabh pandey, peter j. Antioxidant and antiinflammatory activities of extracts. Paper open access phytochemical screening, antioxidant. The use of plants for herbal medicine has been carried.
Fresh ripe pawpaw fruits were bought daily from the ogige market, in nsukka, enugu state nigeria, pilled, washed and blended into juice using an. In some parts of asia, the young leaves of the papaya are steamed and eaten like spinach. Antioxidant properties of carica papaya on cadmium toxicity. Materials and methods food sampling convenience sampling method was used to obtain the samples. Carica pubescens leaves, antioxidant activity, lcms, total phenolic. Here we evaluated the effects of a tropical fruit, unripe carica papaya ucp. Feb 03, 2016 dpph free radical scavenging activity of different extracts of c. This study was conducted to establish polyphenols, flavonoids and antioxidant potential profile of extracts of all major parts of the c. Antiinflammatory and immunomodulatory properties of. Papaya leaves also contain flavonoids, alkaloids phenolic compounds and cynogenetic compounds, and are also reported to be able to treat dengue fever.
Other organic solvents such as the extracts of nhexane, dichloromethane, nbutanol, ethyl acetate, methanol and ethanol showed a significant difference in scavenging activity between the different parts of carica papaya. Evaluation of phenolic content and antioxidant activity of. Pdf antioxidant potential of carica papaya peel and seed. Proximate analysis, antioxidant and antiproliferative activities of. Carica papaya, phytochemicals, antioxidant activity, statistical analysis. These methods are recommended as useful tools for the evaluation of the total activity antioxidant in fruits. Antioxidant analysis of total phenol, total flavonoid, and ferric reducing antioxidant property frap on aqueous extracts of unripe papaya. Effects of drying on total polyphenols content and antioxidant properties of carica papaya leaves jing y yap. Antibacterial and antioxidant activity of plant latex.
An investigation of the antioxidant property of carica papaya. Being a stable free radical, the dpph assay is a simple. Antibacterial activity of seed and leaf extract of carica. Pdf thin layer chromatographic analysis and antioxidant. Pharmacological activity of each division of carica papaya whole carica papaya has a unique pharmacological uses 4. Jun 05, 2012 the carica papaya extract cpe have hepatoprotective effect against carbon tetrachloride intoxicated rats and it may be mediating its protective effects either by decreasing the metabolic activation of carbon tetrachloride, or by acting as a chainbreaking antioxidant for scavenging free radicals or by a combination of these effects. The antioxidant activities were carried out by dpph free. Antioxidant activity of carica papaya seed extracts on. Bioactive compounds and antioxidant activity of fresh. View the article pdf and any associated supplements and figures for a period of 48 hours. The data of active compounds in papaya leaves were analyzed descriptively. Our results indicated promising perspectives for the development and usage of fruits species studied with considerable levels of antioxidant activity.
Phytochemical screening, antimicrobial and antioxidant. One kilogram of fresh papaya carica papaya was purchased from pasar tani masjid jamek, serdang while five hundred grams of pickled. Materials and methods materials dpph 2,2diphenyl1picrylhydrazyl, gallic acid, quercetin, beta carotene were purchased from sigmaaldrich mo, usa, papaya carica papaya, ethanol. One such botanical source that has been reported to possess therapeutic effects is the papaya fruit webman et al. These isolates were selected from carica papaya leaves have antioxidant and antibacterial potential of secondary metabolite. Pdf an investigation of the antioxidant property of carica papaya. Antioxidant properties of unripe carica papaya fruit extract and its. Antioxidant and apoptotic activity of papaya peel extracts. The methanolic extract of carica papaya latex gave the widest zone of inhibition. Carica papaya has admirable restorative properties for treatment of various diseases. Pdf antioxidant activity of seed extracts of annona. Study of phytochemical, elemental analysis and antioxidant. Antioxidant properties of unripe carica papaya fruit extract.
In vitro phytochemical screening and antioxidant activity. Sahira banu and others published antioxidant activity of carica papaya leaf extract rajasthan variety. Pawpaw seeds from riped pawpaw carica papaya fruits was obtained from asmau farms, along gombi road, jimeta. The present study evaluates the in vitro antioxidant activity of eleven different fruits viz.
The carica papaya extract cpe have hepatoprotective effect against carbon tetrachloride intoxicated rats and it may be mediating its protective effects either by decreasing the metabolic activation of carbon tetrachloride, or by acting as a chainbreaking antioxidant for scavenging free. The objective of this current study was to evaluate the antioxidant properties of carica papaya extract in cadmium induced prefrontal cortex toxicity through a critical measurement of antioxidant enzymes activities. Pusa dwarf linn jyotsna kiran peter, yashab kumar, priyanka pandey and harison masih department of microbiology and fermentation technology, jsbb, shiats deemed to be university, naini, up, india 211008. We found that the antioxidant activity of vitamin c and carica papaya seeds extract is high when added during the recovery after oxidative stress for vitamin c and during oxidative stress for carica papaya seeds extract. The tree is always with a soft main trunk and tufted leaves at the top. Khokhardistribution of conjugated and free phenols in fruits. In previous studies, the antioxidant activities of carica papaya extracts reported are lowest than our results 32. Preparation of sample flesh of carica papaya which were.
Hewavitharana school of pharmacy, the university of queensland. Comparative and synergistic antioxidant properties of carica papaya leaf cpl and azadarichta indica leaf ail, which are popularly used as medicinal plants were evaluated by analyzing the individual leaves and their 1. Patients were advised to eat papaya fruit frequently during dengue fever epidemic in pakistan by physicians. Carica papaya, phytochemicals, antioxidant activity, statistical analysis introduction carica papaya has admirable restorative properties for treatment of various diseases. Research article administration dependent antioxidant. Papaya fruits contain vitamins, minerals, enzymes, proteins, carotenoids and polyphenols 21 22. Characterization and antioxidant evaluation of phenolic.
Research article administration dependent antioxidant effect. Compositional difference in antioxidant and antibacterial. Materials and methods preparation of plant material. It has been proven that high consumption of fruit and vegetable lowers the risks of cardiovascular and other oxidative stressrelated diseases. Antioxidant analysis of different parts of carica papaya abstract this study was conducted to compare the total antioxidant activity taa, total phenolic content tpc and total flavonoid content tfc from the different parts of papaya tree including their ripe and unripe fruit, seeds and the young leaves. Tpc was determined by folinciocalteu method, while antioxidant. The antioxidant effect of fermented papaya preparation in. Jul 20, 2010 the purpose of this paper is to investigate various extracts of annona squamosa l annonanceae and carica papaya l caricaceae seeds for their antioxidant activity, free radical scavenging ability, total phenolic and flavonoid contents. Antioxidant and gelatinolytic activities of papain from. This study was therefore aimed at evaluating the antioxidant activity of c. Antioxidant and apoptotic activity of papaya peel extracts in.
Influence of processing in two cultivar of carica papaya. Carica papaya, solo, solomix, antimicrobial, antioxidant 1. Antioxidant analysis of different parts of carica papaya abstract this study was conducted to compare the total antioxidant activity taa, total phenolic content tpc and total flavonoid content tfc. Bioactive compounds and antioxidant activity of fresh exotic. Carica papaya, aerial parts, anticancer activity, phytoconstituents. The fruits, harvested with more than 50% of yellow surface between 60% and 77% were tested for carotenoids content, phenolic content, reducing activity abts and cellular. The ethyl acetate fraction showed the strongest dpph and hydroxyl free radicalscavenging activities, and its activities. Carica papaya fruit, acrylamide, oxidative stress, immunoglobulin. Antioxidant activity of carica papaya leaf extract rajasthan. Antioxidant properties of carica papaya on cadmium.
Therefore, the present investigation has been carried out to find out the antioxidant activity of c. Antioxidant and immunostimulant effect of carica papaya. Papaya seeds exhibited invitro antioxidant activity. Other organic solvents such as the extracts of nhexane, dichloromethane, nbutanol, ethyl acetate, methanol and ethanol showed a significant difference in scavenging activity between the different parts of carica papaya 32 39. Araliaceae to evaluate their antioxidant activities compared to the antioxidant enzyme present in humans, the superoxide dismutase sod. Antioxidant activity of seed extracts of annona squamosa. This study demonstrates the efficacy of ethanolic leaf extracts of c. So the dpph free radicalscavenging activity of the ethyl acetate fraction.
The objective of this current study was to evaluate the antioxidant properties of carica papaya extract in cadmium induced prefrontal cortex toxicity through a critical measurement of antioxidant enzymes activities in the brain tissue. The objective of this study was to determine invitro antioxidant and apoptotic activity. Drying methods had significant p antioxidant phytochemicals, that is, total phenol, tannin, total flavonoid, total carotenoid, and vitamin c levels of the seeds, with freezedrying. The antioxidant properties of ucp were investigated using the assays of frap. The antioxidant properties were determined by 2,2diphenyl1picrylhydrazyl dpph, ferric reducing antioxidant power, and metal chelating assay methods. Therefore, in this study, radical scavenging activity was determined for the selected parts of papaya plant. The distinctive parts of carica papaya plant including leaves, seeds, latex and natural product shown to have therapeutic worth 1. Antioxidant activity using dpph free radical scavenging assay indicated leaves, bark, roots and pulp extracts showed 75. Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern brazil. The results from this study can be used to evaluate these protease enzymes for hypertrophic scar or keloid treatment.
Phytochemical screening, antioxidant activity, and. In some parts of asia, the young leaves of the papaya. Paper open access antioxidant and antibacterial activities of. Thus, any plant possessing antioxidant activity could be a potential lead for curing any of the above ailments. Anticancer activity of carica papaya extracts in and. The antioxidant activities of the ethanol, petroleum ether, ethyl acetate, nbutanol and water extract fractions from the seeds of papaya were evaluated in this study. Pdf antioxidant activity of papaya seed extracts researchgate. Ec3 of secondary metabolite has the free radical scavenging activity of 68.
The present study was undertaken to analyse the thin layer chromatography and antioxidant activities of methanol extract of leaves of carica papaya. Antioxidant activity of seed extracts of annona squamosa and. Nutraceutical potential of carica papaya in metabolic syndrome. Oxidative damage caused by free radicals has major implications in many chronic diseases. Antioxidant activity of carica papaya leaf extract rajasthan variety. Phytochemical screening confirmed the presence of antioxidant, flavonoids and phenols. Papaya seed extracts induced endogenous antioxidant enzymes in hepg2 cells. Phytochemical and antioxidant analysis of aqueous extracts 70 table 2. Agronomy free fulltext physicochemical, nutraceutical. Whole fruit extract contains ferulic, pcoumaric, and ca eic acid, carotenoids, and vitamin c that.
We found that the antioxidant activity of vitamin c and carica papaya seeds extract is high when added during the recovery after oxidative stress for vitamin c and during oxidative stress for carica papaya. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Diabetes the killer disease known worldwide has been implicated to generate oxidative stress and carica papaya has been suggested to manage this effect. Research article invitro antioxidant activities of vitex.
The antioxidant activities of solo and solomix showed ic50 of 1. Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. Antioxidant properties of unripe carica papaya fruit. Antioxidant activity of fermented papaya preparation fpp in the absence and presence of saliva. Antioxidant activity of papaya seed extracts against h2o2. Ucp processed high antioxidant activity and scavenging activity against h 2 o 2.
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